org.biojava3.core.sequence
Class GeneSequence

java.lang.Object
  org.biojava3.core.sequence.template.AbstractSequence<NucleotideCompound>
      org.biojava3.core.sequence.DNASequence
          org.biojava3.core.sequence.GeneSequence

All Implemented Interfaces:

Iterable<NucleotideCompound>, Accessioned, Sequence<NucleotideCompound>

public class GeneSequence
extends DNASequence

Author:

Scooter Willis

Nested Class Summary

Nested classes/interfaces inherited from class org.biojava3.core.sequence.DNASequence

DNASequence.DNAType

Nested classes/interfaces inherited from class org.biojava3.core.sequence.template.AbstractSequence

AbstractSequence.AnnotationType

Constructor Summary

GeneSequence(ChromosomeSequence parentSequence, int begin, int end, Strand strand)
A class that keeps track of the details of a GeneSequence which is difficult to properly model.

Method Summary

ExonSequence	addExon(AccessionID accession, int begin, int end) Add an ExonSequence mainly used to mark as a feature
IntronSequence	addIntron(AccessionID accession, int begin, int end) Add an Intron Currently used to mark an IntronSequence as a feature
TranscriptSequence	addTranscript(AccessionID accession, int begin, int end) Add a transcription sequence to a gene which describes a ProteinSequence
ArrayList<ExonSequence>	getExonSequences() Get the exons as an ArrayList
ArrayList<IntronSequence>	getIntronSequences() Get the introns as an ArrayList
ChromosomeSequence	getParentChromosomeSequence() The parent ChromosomeSequence which contains the actual DNA sequence data
DNASequence	getSequence5PrimeTo3Prime() Try to give method clarity where you want a DNASequence coding in the 5' to 3' direction Returns the DNASequence representative of the 5' and 3' reading based on strand
Strand	getStrand() A gene should have Strand
TranscriptSequence	getTranscript(String accession) Get the transcript sequence by accession
LinkedHashMap<String,TranscriptSequence>	getTranscripts() Get the collection of transcription sequences assigned to this gene
ExonSequence	removeExon(String accession) Remove the exon sequence
IntronSequence	removeIntron(String accession) Remove the intron by accession
TranscriptSequence	removeTranscript(String accession) Remove the transcript sequence from the gene
void	setStrand(Strand strand)
void	validate() Once everything has been added to the gene sequence where you might have added exon sequences only then you can infer the intron sequences and add them.

Methods inherited from class org.biojava3.core.sequence.DNASequence

getComplement, getDNAType, getGCCount, getReverse, getReverseComplement, getRNASequence, getRNASequence, getRNASequence, getRNASequence, main, setDNAType

Methods inherited from class org.biojava3.core.sequence.template.AbstractSequence

addFeature, addFeature, addNote, countCompounds, getAccession, getAnnotationType, getAsList, getBioBegin, getBioEnd, getCompoundAt, getCompoundSet, getDatabaseReferences, getDescription, getFeatures, getFeatures, getFeatures, getFeaturesByType, getFeaturesKeyWord, getIndexOf, getInverse, getLastIndexOf, getLength, getNotesList, getOriginalHeader, getParentSequence, getProxySequenceReader, getSequenceAsString, getSequenceAsString, getSequenceScore, getSource, getSubSequence, getTaxonomy, getUserCollection, iterator, removeFeature, removeNote, setAccession, setAnnotationType, setBioBegin, setBioEnd, setCompoundSet, setDatabaseReferences, setDescription, setFeaturesKeyWord, setNotesList, setOriginalHeader, setParentSequence, setProxySequenceReader, setSequenceScore, setSource, setTaxonomy, setUserCollection, toString

Methods inherited from class java.lang.Object

clone, equals, finalize, getClass, hashCode, notify, notifyAll, wait, wait, wait

Constructor Detail

GeneSequence

public GeneSequence(ChromosomeSequence parentSequence,
                    int begin,
                    int end,
                    Strand strand)

A class that keeps track of the details of a GeneSequence which is difficult to properly model. Two important concepts that is difficult to make everything flexible but still work. You can have GFF features that only describe Exons or Exons/Introns or CDS regions and one or more Transcriptions. You can have exon sequences but that does not imply transcription to the actual protein. The GeneSequence will keep track of Exons and Introns but to get a Protein sequence you need to start with a TranscriptSequence and then add CDS sequences. This is also a key class in the biojava-3-genome module for reading and writing GFF3 files

Parameters:

parentDNASequence -

begin -

end - inclusive of end

strand - force a gene to have strand and transcription sequence will inherit

Method Detail

getParentChromosomeSequence

public ChromosomeSequence getParentChromosomeSequence()

The parent ChromosomeSequence which contains the actual DNA sequence data

Returns:

validate

public void validate()

Once everything has been added to the gene sequence where you might have added exon sequences only then you can infer the intron sequences and add them. You may also have the case where you only added one or more TranscriptSequences and from that you can infer the exon sequences and intron sequences. Currently not implement

getStrand

public Strand getStrand()

A gene should have Strand

Returns:

the strand

setStrand

public void setStrand(Strand strand)

Parameters:

strand - the strand to set

getTranscript

public TranscriptSequence getTranscript(String accession)

Get the transcript sequence by accession

Parameters:

accession -

Returns:

getTranscripts

public LinkedHashMap<String,TranscriptSequence> getTranscripts()

Get the collection of transcription sequences assigned to this gene

Returns:

removeTranscript

public TranscriptSequence removeTranscript(String accession)

Remove the transcript sequence from the gene

Parameters:

accession -

Returns:

addTranscript

public TranscriptSequence addTranscript(AccessionID accession,
                                        int begin,
                                        int end)
                                 throws Exception

Add a transcription sequence to a gene which describes a ProteinSequence

Parameters:

accession -

begin -

end -

Returns:

Throws:

Exception - If the accession id is already used

removeIntron

public IntronSequence removeIntron(String accession)

Remove the intron by accession

Parameters:

accession -

Returns:

addIntron

public IntronSequence addIntron(AccessionID accession,
                                int begin,
                                int end)
                         throws Exception

Add an Intron Currently used to mark an IntronSequence as a feature

Parameters:

accession -

begin -

end -

Returns:

Throws:

Exception

removeExon

public ExonSequence removeExon(String accession)

Remove the exon sequence

Parameters:

accession -

Returns:

addExon

public ExonSequence addExon(AccessionID accession,
                            int begin,
                            int end)
                     throws Exception

Add an ExonSequence mainly used to mark as a feature

Parameters:

accession -

begin -

end -

Returns:

Throws:

Exception

getExonSequences

public ArrayList<ExonSequence> getExonSequences()

Get the exons as an ArrayList

Returns:

getIntronSequences

public ArrayList<IntronSequence> getIntronSequences()

Get the introns as an ArrayList

Returns:

getSequence5PrimeTo3Prime

public DNASequence getSequence5PrimeTo3Prime()

Try to give method clarity where you want a DNASequence coding in the 5' to 3' direction Returns the DNASequence representative of the 5' and 3' reading based on strand

Returns: