Module org.scijava.ops.flim

Package org.scijava.ops.flim

Class Pseudocolor

java.lang.Object

org.scijava.ops.flim.Pseudocolor

All Implemented Interfaces:

Functions.Arity6<FitResults,Float,Float,Float,Float,ColorTable,Img<ARGBType>>

public class Pseudocolor
extends Object
implements Functions.Arity6<FitResults,Float,Float,Float,Float,ColorTable,Img<ARGBType>>

Generates an HSV pseudocolored image of FLIM fit results, where for each pixel:

• The Hue is a function of the lifetime fit parameters
• The Value is a function of the initial intensity fit parameters

Author:

Dasong Gao, Gabriel Selzer

Implementation Note:

op names="flim.pseudocolor"

Constructor Summary

Constructors

Constructor	Description
Pseudocolor()

Method Summary

Modifier and Type	Method	Description
Img<ARGBType>	apply(FitResults rslt, Float cMin, Float cMax, Float bMin, Float bMax, ColorTable lut)
static ColorTable8	spci()	ColorTable used by the SPCI application
static ColorTable8	tri2()	ColorTable used by the TRI2 application

Methods inherited from class java.lang.Object

clone, equals, finalize, getClass, hashCode, notify, notifyAll, toString, wait, wait, wait

Methods inherited from interface org.scijava.function.Functions.Arity6

andThen

Constructor Detail

Pseudocolor

public Pseudocolor()

Method Detail

apply

public Img<ARGBType> apply(FitResults rslt,
                           Float cMin,
                           Float cMax,
                           Float bMin,
                           Float bMax,
                           ColorTable lut)

Specified by:

apply in interface Functions.Arity6<FitResults,Float,Float,Float,Float,ColorTable,Img<ARGBType>>

Parameters:

rslt - the results from the Fit

cMin - The lower bound used for the hue map. Hue values below this bound will be mapped to black. Defaults to the fifth percentile value across all lifetime values.

cMax - The upper bound used for the hue map. Hue values above this bound will be mapped to white. Defaults to the ninety-fifth percentile value across all lifetime values.

bMin - The lower bound used for the value map. Defaults to the minimum of rslt.intensityMap

bMax - The upper bound used for the value map. Defaults to the 99.5th percentile of rslt.intensityMap

lut - the ColorTable function to map hues between cMin and cMax. Defaults to tri2().

Returns:

tri2

public static ColorTable8 tri2()

ColorTable used by the TRI2 application

Returns:

a ColorTable that colors images similar to the TRI2 application

spci

public static ColorTable8 spci()

ColorTable used by the SPCI application

Returns:

a ColorTable that colors images similar to the SPCI application